Normally this pressure is 0.25 inches of water column, but could be raised or decreased as the procedure requires. Isolators need to be periodically leak tested to make certain their integrity and forestall escape from the decontamination agent. Leak testing can be done by different approaches together with pressure https://www.tumblr.com/tailinscitech/805882763435802624/enhancing-lab-efficiency-with-tailin-toc-analyzer?source=share