The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR merchandise have been digested and cloned right into a pET21a+ vector utilizing NdeI and XhoI restriction enzymes. A degree mutation was launched by the short https://zaneapygm.dreamyblogs.com/26908359/agx51-for-dummies